Comparing COL7A1 gene expression in fibroblast cells of dystrophic epidermolysis bullosa patients with clinical responses to autologous fibroblasts transplantation

Objectives: This clinical research aimed to establish autologous fibroblasts transplantation as a possible treatment for patients with DEJ. The COL7A1 gene expression was also evaluated.
 Methods: Six (3M and 3F), 4 no recurrent wounds 2 after surgery, 15 healthy subjects were included in the study controls. Quantitative real-time polymerase chain reaction (real-time PCR) analysis of performed using an oligonucleotide primer pair designed amplify across exon/exon junction.
 Results: level down-regulated at exons 26-27, 47-48, 96-97, 116-117 all patients′ compared However, positive response not significantly changed poor response. (ClinicalTrials.gov NCT01908088)
 Conclusion: In this mRNA levels less when However different between two groups patients, further examination is needed elucidate mechanism treatment.